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. 2012 Jun 15;287(32):26740–26748. doi: 10.1074/jbc.M112.383265

FIGURE 7.

FIGURE 7.

Nrdp1 promotes transcriptional activity of C/EBPβ. A, NIH-3T3 cells were co-transfected with Arg1 luciferase reporter plasmid, pRL-TK-Renilla-luciferase plasmid, and the indicated amounts of pcDNA3.1-Nrdp1, with or without pcDNA3.1-C/EBPβ. After 24 h, the cells were stimulated with IL-4 (10 ng/ml). Arg1 reporter activity in lysates was measured by Dual-Luciferase reporter assay system. B, Western blot and Q-PCR validation of siRNA-C/EBPβ (si-C/EBPβ) silencing efficiency of C/EBPβ. C, NIH-3T3 cells were co-transfected with Arg1 luciferase reporter plasmid, pRL-TK-Renilla-luciferase plasmid, and pcDNA3.1-Nrdp1 with si-C/EBPβ or scrambled control siRNA (si-Ctrl). After 24 h, the cells were stimulated with IL-4 (10 ng/ml). Arg1 reporter activity in lysates was measured, and luciferase activity was normalized to Renilla luciferase activity and was presented relative to basal luciferase activity. Data are the mean ± S.D. of five samples in one experiment representative of similar results obtained in three independent experiments. *, p < 0.05; **, p < 0.01.