FIGURE 9.

PrkD-dependent phosphorylation of BasPyk in E. coli and effect of phosphorylation on BasPyk activity. A, phosphospecific staining of equal amounts of phosphorylated and unphosphorylated forms of BasPyk by ProQ Diamond stain (top). Protein amounts were verified by Sypro Ruby staining (bottom). B, histogram comparing the specific activity of Pyk-P and Pyk-UP in units/mg of protein/min. Phosphorylated and unphosphorylated forms of BasPyk were compared in identical conditions. The experiment was performed three times, and the error bars represent S.D. of three individual values.