FIGURE 2.

Mutations of His¹⁴⁷, His¹⁴⁹, and His¹⁵¹ decrease secreted sAPPα levels. HEK293 cells stably overexpressing WT-APP or APP mutants (single, double, and triple mutants) were cultured under standard conditions. Conditioned cell culture media were examined by Western blot technique using WO2 antibody to detect sAPPα metabolite. Densitometry values were normalized to intracellular APP levels of the corresponding lysates and expressed as a percentage relative to that of WT-APP (100%). Data are expressed as mean ± S.E. (error bars). N indicates the number of times the experiment was performed. Statistical significance values were calculated with one-way ANOVA using Dunnett's post hoc test comparing each mutant with the WT-APP control. **, p < 0.01.