TABLE 2.

Kinetics and affinity constants for wild-type and mutant Tsi1 proteins binding to Tse1

Protein	Association rate (ka)	Dissociation rate (kd)	Binding affinity (Ka)
	m−1 s−1	s−1	m
WT	7.32 × 105	2.03 × 10−3	2.77 × 10−9
S107A	4.57 × 102	1.83 × 10−3	4.00 × 10−6
E65A	8.14 × 104	3.42 × 10−3	4.20 × 10−8
I86A	8.00 × 104	2.44 × 10−3	3.04 × 10−8
F106A	6.12 × 105	8.04 × 10−3	1.31 × 10−8
S109A	6.14 × 105	2.04 × 10−3	3.33 × 10−9
D61A	6.08 × 105	2.54 × 10−3	4.17 × 10−9
E150A	6.53 × 105	2.35 × 10−3	3.61 × 10−9