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1H NMR spectra.A, holo-Y75A at pH 5.6; B, holo-Y75A at pH 8.2. C, holo-Y75A cyanide adduct at pH 7.3; D, holo-H32A at pH 5.6; E, holo-H32A at pH 9.1. Spectra were recorded at 303 K, in 20 mm phosphate buffer.