TABLE 2.
Bulk susceptibility shifts (Δδ, ppm) for H32A and WT HasA measured against 1,4-dioxane by the Evans method in the temperature range 283–308 K at pH 5.7 and 800 MHz
The resulting paramagnetic contribution to the molar susceptibility (χMPARA, m3 mol−1 × 108) and the effective magnetic moment (μeff, μB) of the paramagnetic center are also reported.
| T | H32A |
WT |
||||
|---|---|---|---|---|---|---|
| Δδ | χMPARA | μeff | Δδ | χMPARA | μeff | |
| K | ppm | (m3 mol−1 × 108) | μB | ppm | (m3 mol−1 × 108) | μB |
| 283 | 19.9 | 8.91 | 3.86 | 11.7 | 5.23 | 3.02 |
| 288 | 19.3 | 8.64 | 3.88 | 12.4 | 5.56 | 3.11 |
| 293 | 19.0 | 8.51 | 3.88 | 12.9 | 5.78 | 3.20 |
| 298 | 18.6 | 8.33 | 3.87 | 13.6 | 6.09 | 3.31 |
| 303 | 18.2 | 8.15 | 3.86 | |||
| 308 | 17.8 | 7.97 | 3.85 | 13.9 | 6.22 | 3.40 |