FIGURE 1.

BOXSHADE alignment of cyclotide precursors in genus Petunia. A, PETUNITIDE sequences. B, consensus sequences derived from matched ESTs. Asterisks at the C termini of sequences indicate stop codons. Red bar denotes predicted signal motifs, and green bars denote cyclotide or acyclotide domains. Triangles denote prototerminal amino acids of encoded cyclotides. Disulfide connectivity is based upon previously characterized cyclotides. Sequences translated from EST data only are listed in supplemental Table S1 (note paired clones (F + R)).