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. 2012 Jun 12;287(32):27148–27157. doi: 10.1074/jbc.M112.388843

FIGURE 3.

FIGURE 3.

Inhibition of EGFR down-regulated Tyr-211 phosphorylation and the level of chromatin-bound PCNA. MDA-MB-468 cells were treated with EGFR inhibitors lapatinib (10 μm for 24 h) (A–C) or AG1478 (10 μm for 16 h) (D–F). A and D, the endogenous PCNA was immunoprecipitated (IP) by the anti-Tyr(P)-211 PCNA. The immunoprecipitated complexes were examined by Western blot (WB) analysis using an anti-PCNA antibody. Quantitated result of data derived from three independent experiments is shown. **, p < 0.01; *, p < 0.05. B and E, after the treatment of the EGFR inhibitors, the levels of EGFR, Tyr(P)-1068 EGFR (p-EGFR), and α-tubulin are shown. Quantitated result of data derived from three independent experiments is shown. ***, p < 0.005. C and F, the treated cells were extracted with 5% of Triton X-100, and the levels of PCNA in the Triton-resistant and Triton-soluble fractions were determined by Western blot analysis. Histone H3 and α-tubulin were used as the internal control for Triton-resistant and Triton-soluble fractions, respectively. Quantitated result of data derived from three independent experiments is shown. *, p < 0.05; **, p < 0.01. Error bars, S.E.