FIGURE 9.

Inhibitors of autolysis limit pneumococcal biofilm formation. D39 was seeded into biofilms over a prefixed substratum of NCI-H292 cells in chemically defined medium. At each media change, PBS (negative control), 30 μm RuR, or 100 μm DCB was added so that it was present throughout the 48-h growth period. The data represent the total biomass of three individual biofilms per sample after 48 h of incubation, with S.D. indicated with error bars and significance calculated using the paired t test with a 95% confidence interval (* = p < 0.05; ** = p < 0.01).