FIGURE 3.

Western blot analysis of LFA-1/ICAM-1-mediated change in the expression levels of SMAD7, SMURF2, and SKI proteins. PBL T-cells purified from healthy volunteers (a) or Hut78 T-cells (b) were incubated on ICAM-1-coated plates for 1, 3, 6, 12, or 24 h and lysed. c, serum-starved PBL T-cells were pretreated with 10 μm AG490, 50 μm STAT3-specific inhibitory peptide (ST3 pep), or 10 μm JNK inhibitor SP600125 (JNK-I) for 1 h and then incubated on ICAM-1-coated plated for additional 6 h. Cell lysates (20 μg each) were Western blotted and probed with anti-SMAD7, anti-SMURF2, or anti-SKI. Blots were reprobed with anti-α-tubulin or anti-β-actin as a loading control. Relative densitometric analysis of the individual protein band is presented. Data are representative of three independent experiments (mean ± S.E.). *, p < 0.05 with respect to corresponding controls.