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. 2012 Jun 22;31(15):3363–3374. doi: 10.1038/emboj.2012.169

Figure 2.

Figure 2

Age-related accumulation of Sykki marginal zone B cells. (A) B220+ pre-gated splenic cells were stained with anti-IgM and anti-IgD to delineate transitional and mature B cell fractions. (B) Statistical analysis of total B220+, transitional (T) 1/marginal zone (MZ), T2 and mature (M) splenic B cell and (C) CD4+ and CD8+ (CD3+-pre-gated) T cell numbers in ctrl and ki mice. (D) Numbers of (CD3+-pre-gated) CD44hiCD62Llo effector/memory CD4+ and CD8+ T cell subsets and (E) ratio of splenic CD4+ effector/memory T versus CD4+CD25+FoxP3+ nTreg cells. (F, left) Representative dot plot of MZ (B220+CD21hiCD23lo/) and follicular (FO)/T2 (B220+CD21intCD23+) B cells of ctrl mice at the age of 8 weeks (W); numbers in dot plots represent average percentages of B cell subsets. (F, right, G) Age-related accumulation of B220+CD21hiCD23lo/MZ B cells in ki mice. (H) Pre-immune serum immunoglobulin (Ig) titres of ctrl and ki mice. For (AE, H), mice were analysed at the age of 8–12 weeks. *P<0.05, **P<0.01, ***P<0.001; n=7 (A, B), 4 (CG) and 8 (H) per group/age; error bars indicate means±s.e.m. ctrl, control; M, mature; ns, not significant.