Figure 2.

Age-related accumulation of Syk^ki marginal zone B cells. (A) B220⁺ pre-gated splenic cells were stained with anti-IgM and anti-IgD to delineate transitional and mature B cell fractions. (B) Statistical analysis of total B220⁺, transitional (T) 1/marginal zone (MZ), T2 and mature (M) splenic B cell and (C) CD4⁺ and CD8⁺ (CD3⁺-pre-gated) T cell numbers in ctrl and ki mice. (D) Numbers of (CD3⁺-pre-gated) CD44^hiCD62L^lo effector/memory CD4⁺ and CD8⁺ T cell subsets and (E) ratio of splenic CD4⁺ effector/memory T versus CD4⁺CD25⁺FoxP3⁺ nTreg cells. (F, left) Representative dot plot of MZ (B220⁺CD21^hiCD23^lo/−) and follicular (FO)/T2 (B220⁺CD21^intCD23⁺) B cells of ctrl mice at the age of 8 weeks (W); numbers in dot plots represent average percentages of B cell subsets. (F, right, G) Age-related accumulation of B220⁺CD21^hiCD23^lo/−MZ B cells in ki mice. (H) Pre-immune serum immunoglobulin (Ig) titres of ctrl and ki mice. For (A–E, H), mice were analysed at the age of 8–12 weeks. *P<0.05, **P<0.01, ***P<0.001; n=7 (A, B), 4 (C–G) and 8 (H) per group/age; error bars indicate means±s.e.m. ctrl, control; M, mature; ns, not significant.