Figure 2.

Venn diagram showing the number of overlapping proteins identified in human cornea by (i) SDS-PAGE followed by in-gel trypsinization, (ii) in-solution digest without prior protein separation or (iii) in-solution digest followed by cation exchange chromatography. A total of 2958 unique proteins were identified by SDS-PAGE separation, 1010 by in-solution digest without prior protein separation and 1729 by in-solution digest followed by cation exchange chromatography.