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. 2012 Aug 1;2(8):865–871. doi: 10.1534/g3.112.002782

Figure 1 .

Figure 1 

Increased variation in anaphase onset when dosage of CYB-3 is tripled using the MosSCI insertion method. (A) Schematic drawing of the cyb-3 duplication locations in the C. elegans genome. such-4(h2168) is a tandem duplication that affects the region between the T26H8.1 and C06B8.5 nearly identical transposons. dotSi100 is a cyb-3 duplication that was inserted into the ttTi5605 Mos1 insertion site on chromosome II between the F14E5.1 and the F14E5.2 genes. dotSi110 is a cyb-3 duplication that was inserted into the cxTi10882 Mos1 integration site on chromosome IV between the F30B5.1 and the F36E4.9 genes. (B) Summary of the timing measurements from NEBD-to-metaphase and metaphase-to-anaphase intervals given in seconds. Error bars represent SEM for n = 20 measurements for each strain. (C) Boxplots of the metaphase-to-anaphase interval timing data for the embryos with one, two, or three copies of the cyb-3 in the wild-type background (right) and the mdf-1(gk2) background (left). For each boxplot, all the data points (n = 20) were used. (D) dotSi100; dotSi110: An example of an embryo with normal timing of the anaphase onset (140 sec) and delayed anaphase onset (345 sec) in the wild-type background. mdf-1 such-4; dotSi100: An example of an embryo with normal timing of the anaphase onset (195 sec) and delayed anaphase onset (430 sec) in the mdf-1(gk2) background.