Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Aug 1;2(8):943–959. doi: 10.1534/g3.112.003376

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2012 Doherty et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Pulsed-field gel electrophoretic analyses comparing chromosomal damage and killing after no treatments and after 30-min phleomycin treatments. Diploid genotypes with respect to BLM10 and BLM3 were BLM10/BLM10, BLM3/BLM3; blm10Δ/blm10Δ, BLM3/BLM3; and BLM10/BLM10, blm3-1/blm3-1. Treated populations were divided and incubated under nongrowing conditions for 24 or 48 hr, during which competent strains can reconstruct their chromosomes (Moore et al. 2000). Routine microscopic examination and visual counting of cells before and after these LH periods confirmed cell populations did not bud or grow, and cell lysis was never observed. (A) Lanes 1, 4, 7, and 10, no LH. Lanes 2, 5, 8, and 11, 24-hr LH. Lanes 3, 6, 9 and 12, 48-hr LH. (B) Lanes 1, 4, 7, no LH. Lanes 2, 5, 8, 24-hr LH. Lanes 3, 6, 9, 48-hr LH. Corresponding survival data: squares, 0 LH; inverted triangles, 24-hr LH; triangles, 48-hr LH. Pulsed-field gel electrophoreses and survival analyses are representative of three independent experiments and of multiple diploid constructions of the same genotypes.