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. 2012 Aug 1;2(8):943–959. doi: 10.1534/g3.112.003376

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Copyright © 2012 Doherty et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Resistance conferred by truncated Blm10. Expression of Blm10_(-339aa)-GST from an inducible pCUP1 promoter was controlled by adjusting the amounts of copper added to media (Mascorro-Gallardo et al. 1996). Top: blm10Δ cells expressing BLM10. Middle: blm10Δ cells not expressing BLM10 or GST. Bottom: blm10Δ cells expressing GST but not BLM10. Left: cell densities during growth. Right: survival. Green rectangles indicate no copper or phleomycin; orange triangles, 50 μM copper, no phleomycin; inverted pink triangles, 5 μg/mL phleomycin, no copper; black diamonds, 50 μM copper, 5 μg/mL phleomycin.