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. 2012 Aug 1;2(8):943–959. doi: 10.1534/g3.112.003376

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Copyright © 2012 Doherty et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Blm10_(-339aa) localization. The truncated Blm10 protein was induced with 50 μM copper because this concentration produced a stable protein and functionally relieved drug hypersensitivity. The representative wild-type cells illustrate two populations of cells, one unbudded (A) and the other budded (B and C). The nuclear localization is maintained in unbudded cells, in contrast to the bud-neck localization in budded cells. Top row: phase contrast. Middle row: DAPI staining of DNA. Bottom row: cells after GST antibody staining. Column A: unbudded cells. Columns B and C: different populations of budded cells. Column D: cells expressing GST but not Blm10. Column E: no antibody treatment. Because of the truncated protein, these cells are somewhat distended. In addition, before treatments with DAPI and anti-GST antibody, cells are converted to spheroplasts, which causes distortion of cells that have lost their cell wall integrity.