Table 3. Oligonucleotide primers.

Primer Name	Primer Use	Length (Bases)	Sequence 5′-3′
1	Sequencing	20	ATATGCCGCAGACGGAAGAC
2	Sequencing	20	ATATAAGACTGAAAGTCATG
3	Sequencing	21	GCCTATCGTTACATCCGTTGT
4	Sequencing	21	AGTAATTCGGTTTATTGTGAT
5	Sequencing	19	CAAAGAACAAATCAAAAGA
6	Sequencing	20	TCAGTGGCACGTACCTTCTA
7	Sequencing	21	CTTCATTGACGTTGATTTCCT
8	Sequencing	22	CAAAAAGAAAAAGCGTGAGTAC
9	Sequencing	22	AAAGCTCAATTTACGTGAGAAT
10	Sequencing	22	GTTGGTATTTGATCACCCATAC
11	Sequencing	21	GTTCGTGCGGCATCCATTTTG
PA-05	Sequencing/deletion verification/YFP verification	30	GCGCGGTACCATTACGCAGAATAATCTATG
YFP-up	YFP cassette	94	TTCAATTGGGATAAGGTCTTGTTAGTAATGGGAAT
			GGGTGATTTGATATCATCGTCATTGTTAGCGGTCAT
			TTTGTACAATTCATCCATACCATG
YFP-down	YFP cassette	82	TTGCATACATAAACTTTATCATTGTTCGTTAGCTAG
			CTTTGCACATTAATTTTTCGATTTGTTACCGCCACGG
			CCGCCAGGG
Deletion primer 1	Replacement cassette	72	ATGATCTCAAACTGCTTCTTAATATAGGCATCCAC
			CTTTTCTGGGACGCTTTTTACTCTTGGCCTCCTCTAG
Deletion primer 2	Replacement cassette	68	CAAATCTACATGTATATACAGATCTATACAGCAA
			TTATAGGATATCTTTCGTTCAGAATGACACG
HIS3 R	Verify deletion	21	CAGACAATCAACGTGGAGGGT
NF	Sequencing	22	ATTCCCATTACTAACAAGACCT
NR	Sequencing	22	ATCGCAATATAAAGATTAACTA
L1F	Sequencing	22	AATCTTATATTGCGATCAGCTC
L1R	Sequencing	24	GATATGATAAGATAGGGCACAAC
L2F	Sequencing	24	GGGATTTTTACTGATGATCAAATG
L2R	Sequencing	25	GATATGATAATGATAGGGCACAAC
L3F	Sequencing	24	TGTTTAACTTCTTTTTGTCACGAA
L3R	Sequencing	25	GATAGGAATGAAAGCGGCTATAGA
L4F	Sequencing	24	AACCTCATCAACGGTATTGTATCT
L4R	Sequencing	24	TATTTCGGTTGTACATAGAGTTGC
L5F	Sequencing	25	ACTCTATGTACAACCGAAATAACTG
L5R	Sequencing	21	AAATATCAATCTGCCGATGTC
L6F	Sequencing	25	AGTGTATGTGTCATTTCCGATCAAG
L6R	Sequencing	23	CATATTCAGTTCGCAGAAACCAG
CF	Sequencing	24	TCATCTGGTTTCTGCGAACTGAAT
CR	Sequencing	25	GTTAGCGACAGCTGGCGAACCTGA