Fig. 2. TLR3 activation induced IFN-λ expression.
Human primary astrocytes were treated with PolyI:C (10μg/ml), Imiquimod (1μg/ml) or ODN2006 (5μM) for 4h or 24h. RNA extracted from cells was subjected to the real-time RT-PCR for IFN-λ1 and IFN-λ2/3. Data is expressed as IFN-λ mRNA ratio to the internal standard GAPDH. Value is the mean ± SD of three independent experiments (*, p<0.05; **, p<0.01).