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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Neurosci Res. 2011 Nov 4;90(2):399–406. doi: 10.1002/jnr.22758

Fig. 4. Effect of TLR3 activation on HSV-1 infection.

Fig. 4

Human primary astrocytes were treated with or without PolyI:C as indicated concentrations for 24h prior to HSV-1 infection. HSV-1 replication was quantified at 96h postinfection. (A) Intracellular HSV-1 gD gene expression was measured using GAPDH as an internal standard and expressed as ratio to GAPDH expression. (B) Extracellular HSV-1 gD gene expression was examined by using serial dilutions of HSV-1 gD standards with known copy numbers. All value is the mean ± SD of three independent experiments (*, p<0.05; **, p<0.01).