FIG. 2.

Cisplatin-induced S-nitrosylation of cochlear proteins. Western blot analysis with anti-S-nitrosocysteine, 3 days after a 16 mg/kg cisplatin treatment, demonstrated S-nitrosylation of cochlear proteins in Wistar rats. Cisplatin induced significant increases in S-nitrosylation of at least three different cochlear proteins (*p<0.05). The specificity of the antibody detection was verified by the absence of S-nitrosylated protein bands when the membrane was treated with mercuric chloride before immunoblotting (not shown as a similar data is represented in Fig. 3). The immunoblots were normalized with the expression of actin. The numbers indicate the apparent molecular weights. The images are representative samples of three biological replicates and results are expressed as mean±standard error, n=3.