FIG. 1.

The morphology and cell cycle analysis of hLB-MSCs from the 7th and 25th passages. (A, passage 7; B, passage 25). The morphological appearances of hLB-MSCs from different passages are similar. The cells are both spindle-shaped and fibroblast-like. (C and D) 4′,6-Diamidino-2-phenylindole (DAPI) staining of hLB-MSCs. hLB-MSCs with smooth nuclear are positive of DAPI staining. The cells were considered normal on the basis of typical morphology. (E, passage 7; F, passage 25). Cell cycle analysis of hLB-MSCs from the 7th and 25th passages indicated that hLB-MSCs have similar potentials of propagation during long culture in vitro.