FIG. 3.

The mesenchymal differentiation potential of hLB-MSCs. For adipogenic differentiation, small lipid droplets in the cytoplasm could be observed in the cytoplasm stained red with Oil Red after a 14 days of induction. (A) Untreated cells. (B) Phase-contrast photograph (black arrow represents lipid droplets in the cytoplasm). (C) Cells stained positively with Oil Red. For osteogenic differentiation, hLB-MSCs changed from a fibroblast to a cobblestone morphology stained positively for BGLAP. (D) Untreated cells. (E) Phase-contrast picture (cells lost their typical fibroblast appearance turning into a rounder, more cuboidal shape). (F) Cells stained positively with BGLAP.