Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Aug;14(4):364–376. doi: 10.1089/cell.2012.0001

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright 2012, Mary Ann Liebert, Inc.

PMC Copyright notice

FIG. 4. — Specific ESC markers, gene expression, and karyotyping results of putative rbESCs derived and maintained under Condition II. Putative rbESC lines (R4, R9, R15, R21, and R31) derived and maintained under Condition II (REF+rbLIF) are positive for AP (A, top panel) and other pluripotency markers, including Oct-4, Nanog, Sox2, and SSEA4 (B, lower panels). (A) Representative staining images of R4 (passage 7). RT-PCR assays revealed that these cell lines (R4, passage 7; R9, passage 6; R15, passage 4; R21, passage 5; and R31, passage 4) expressed pluripotency genes such as Oct-4, Sox-2, Nanog, c-Myc, Klf4, and Dppa5 (B). GAPDH was used as the internal control. Karyotyping analysis on line R4 (passage 15) and line M5 (passage 15) revealed 57.5% and 60.0% normal ploidy rates, respectively (C). Both R4 (D and E) and M5 (images not shown) were identified as male cell lines. Scale bars, 200 μm.

FIG. 4. — Specific ESC markers, gene expression, and karyotyping results of putative rbESCs derived and maintained under Condition II. Putative rbESC lines (R4, R9, R15, R21, and R31) derived and maintained under Condition II (REF+rbLIF) are positive for AP (A, top panel) and other pluripotency markers, including Oct-4, Nanog, Sox2, and SSEA4 (B, lower panels). (A) Representative staining images of R4 (passage 7). RT-PCR assays revealed that these cell lines (R4, passage 7; R9, passage 6; R15, passage 4; R21, passage 5; and R31, passage 4) expressed pluripotency genes such as Oct-4, Sox-2, Nanog, c-Myc, Klf4, and Dppa5 (B). GAPDH was used as the internal control. Karyotyping analysis on line R4 (passage 15) and line M5 (passage 15) revealed 57.5% and 60.0% normal ploidy rates, respectively (C). Both R4 (D and E) and M5 (images not shown) were identified as male cell lines. Scale bars, 200 μm.