Table 3.
Population Doubling (PD) Time of Putative Rabbit ESC Line R4 (Passage 5) with Different Feeders and LIF Supplementation
| hLIF (h) | mLIF (h) | rbLIF (h) | |
|---|---|---|---|
| REFs | 12.72±0.76a | 14.02±1.74a | 14.00±0.72a |
| MEFs | 15.28±2.95a | 15.06±2.81a | 27.04±1.87b |
a,b
Numbers with different superscripts differ significantly. The PD time was in normal range under both conditions I (hLIF+MEFs, 15.28 h) and II (rbLIF+REFs, 14.00 h); however, the PD time was abnormally long under the rbLIF+MEFs condition (27.04 h).
ESC, embryonic stem cells; LIF, leukemia inhibitory factor; h, human; m, mouse; rb, rabbit; REFs, rabbit embryonic fibroblasts; MEFs, mouse embryonic fibroblasts.