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. Author manuscript; available in PMC: 2012 Aug 3.
Published in final edited form as: Stem Cells. 2009 Sep;27(9):2362–2370. doi: 10.1002/stem.163

Figure 4. Increased β-hexosaminidase enzyme levels in brains of NSC-treated Hexb−/− mice.

Figure 4

(A) Representative Hex levels in serial coronal brain sections of individual hemisphere from NSC-treated and sham-treated Hexb−/− mice at 4 weeks posttransplant. Brain homogenates were made from 12 individual brain slices, as per schematic diagram, and hex levels were measured against 4-MUGlcNAc artificial substrate (mean ± s.e.m., n=3). Ipsilateral represents the injected hemisphere, while contralateral represents the noninjected hemisphere. (B) RT-PCR analysis for β-subunit expression of Hex from local brain regions. Two or more brain regions (dorsal cortex/thalamus/lateral cortex/hippocampus) expressed the Hexβ-subunit gene in each hemisphere, indicative of donor-derived cell migration and brain engraftment upon unilateral (hippocampus) NSC transplantation.