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. Author manuscript; available in PMC: 2012 Aug 3.
Published in final edited form as: Stem Cells. 2009 Sep;27(9):2362–2370. doi: 10.1002/stem.163

Figure 6. Engrafted NSCs differentiate into a range of neural cell types in the Hexb−/− mouse brain.

Figure 6

Following NSC transplantation at 13 weeks of age, brains of Hexb−/− mice were processed for cell type characterization using immunoflourescence staining for both LacZ expressed β-gal and neural cell-type specific markers. At 3 weeks posttransplant, NSCs stably engrafted in the Hexb−/− mouse brain, differentiating into a range of neural cell types. (A–D) Representative donor-derived cells, recognized by their immunoreactivity to β-gal (green, bottom), coexpress neuronal markers such as neurofilament (NF), astroglial marker GFAP, oligodentrocyte marker MBP and nestin, a marker of undifferentiated neural precursors. Merged images show blue DAPI nuclear staining identifies all cells in the field (bottom). Each merged image is also shown as an orthogonal projection composed of 9–16 optical Z-planes, 0.5–1 μm thick. Arrows in each panel of a given column indicate the same cell. Scale bar = 10 μm. (see also Supplementary Fig 2).