FIG. 4.

Trophin expression and protein quantitation for VEGF-A and BDNF are concordant. Concordant RNA (left) and protein (right) quantitation is shown for VEGF (A) and BDNF (B), all analyzed 1 week post-transplant. The expression data have been re-plotted from Figure 3 with an alternate normalization to GAPDH alone. Bands used for densitometry measurements for 22 animals among the 6 experimental groups are shown in (C).

Sample sizes: Uninjured qPCR 3, Western Blot n=3; Injured No Treatment qPCR n=3, Western Blot n=4; Injured + GF/IS qPCR n=3 Western Blot n=3, Dead Cell + GF/IS qPCR n=4, Western Blot n=3, NPC + IS Only qPCR n=3, Western Blot n=3, NPC + GF/IS qPCR n=3 Western Blot n=6. qPCR was performed in quadruplicate. Color images available online at www.liebertonline.com/scd