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. 2012 Jan 9;21(12):2160–2169. doi: 10.1089/scd.2011.0670

FIG. 2.

FIG. 2.

Enhanced proteoglycan and collagen type II deposition by ES-expanded MSC pellets. MSCs of 5 donors were expanded up to passage 3 in 4 different media and MSCs of each medium group were subjected to chondrogenic differentiation for 6 weeks. (A) Proteoglycan deposition was estimated by alcian blue assay and (B) collagen type II deposition was determined by ELISA (each 2 pellets per donor and medium group). Data are given relatively to control medium set as 1. Mean±s.d., *P<0.05 compared with ES and conditioned ES medium. #P<0.05 compared with ES medium (P<0.05, Mann–Whitney U-test).