FIG. 2.

Chimera formation between medaka embryonic stem (ES) cells and zebrafish embryos. (A and B) Micrograph of a zebrafish embryo host, showing transplanted medaka haploid ES cell line HX1 (green; A) and diploid ES cell line MES1 (red; B). (C and D) Micrograph of 1-day-old chimera, showing similar distribution of HX1 (C) and MES1 (D). (E and F) Micrograph of 2-day-old chimeras, showing wide distribution of HX1 (E) and MES1 (F). (G and H) Merged micrograph of a 3-day-old chimera, showing a similarly wide distribution of HX1 and MES1 donor cells. Scale bars, 100 μm. Color images available online at www.liebertonline.com/scd