FIG. 1.

(A and B) The expression of reprogramming transcriptional factors. Quantitative analysis was performed on reprogramming transcriptional factors expression relative to GAPDH in the following cell passages: P0, P5, P10, P15, and P20 of AMSCs and BMSCs. Expression data for each passage were analyzed three times. Vertical bars represent the mean±standard error. (C) Morphological observations and expression of reprogramming transcriptional factors in AMSCs. A–E represent c-Myc, Klf4, Nanog, Oct4, and Sox-2, respectively; A1–E1 are stained with DAPI; A2–E2 are confocal pictures. (D) Morphological observations and expression of reprogramming transcriptional factors in BMSCs. A–E represent c-Myc, Klf4, Nanog, Oct4, and Sox-2, respectively; A1–E1 are stained with DAPI; A2–E2 are confocal pictures. (The fluorescence intensity of coal maceral did not represent protein level).