FIG. 2.

Helios presents a dynamic spatio-temporal pattern of expression during LGE and striatal development. (A) Helios immunohistochemistry was performed in LGE coronal sections at different stages of embryonic (E14.5, E16.5, and E18.5) and postnatal (P3, P7, and P15) development. Helios is initially expressed at the ventro-lateral area of E14.5 mantle zone (MZ LGE and progressively spreads to the dorsomedial area during development. The expression of Helios is reduced at later postnatal stages and only a few positive cells can be observed at P15. (B) Quantification of Helios mRNA levels by Q-PCR during LGE development showing a peak of expression at E18.5. Results are expressed as the mean of 3 to 5 LGE samples and error bars represents the standard error of the mean (SEM). Statistical analysis was calculated by Student's t-test; *P<0.05; **P<0.005 relative to E14.5 expression. Scale bars: (A) 500 μm. (n.d., not detected).