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. 2011 Dec 5;21(12):2239–2251. doi: 10.1089/scd.2011.0607

FIG. 3.

FIG. 3.

Helios is expressed by nestin-positive cells in the SVZ and MAP2-positive cells in the MZ of the LGE. Double immunohistochemistry was performed in mouse brain coronal sections at E16.5 for Helios and β-III-tubulin (Tuj-1) (A), MAP2 (B), and nestin (C). (A, B). Note the expression of Helios in 2 different cell populations: a small one located in the Tuj-1- and MAP2-negative SVZ of the LGE that is spread through the ventral and dorsal part (A–B), and a larger one distributed along the MZ where it colocalizes with the neuronal marker MAP2 (B). (C) Representative high-magnification photomicrograph showing double immunohistochemistry for nestin and Helios both at the SVZ and MZ. Colocalization of both markers is observed in the SVZ of the LGE. Z-analysis shows the colocalization of Helios positive nuclei (arrows) surrounded by nestin-positive intermediate filaments. VZ, ventricular zone; SVZ, subventricular zone; MZ, mantle zone (delimited by dotted area). Scale bars: (B) 150 μm; (C) 100 μm.