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. 2011 Dec 5;21(12):2239–2251. doi: 10.1089/scd.2011.0607

FIG. 4.

FIG. 4.

Helios is expressed by neuronal precursors located in the striatal matrix compartment. Double immunohistochemistry was performed in mouse brain coronal sections at several embryonic and postnatal stages for Helios and striatal neuronal markers such as: DARPP-32 (A), NeuN (B, C), Bcl11b (Ctip2) (D), and Foxp1 (E). Representative photomicrographs show that Helios is not expressed in the striosomal compartment, since it has a complementary distribution with DARPP-32, a striosomal marker at this stage (A1, A2; note that A2 is a high magnification of A1). At P3, Helios does not colocalize with NeuN, a marker of mature neurons that at this stage presents a striosomal distribution (B1, B2; note that B2 is a high magnification of B1). However, both markers are coincident in some cells at P7, developmental stage at which NeuN is staining most of the striatal neurons (C1, C2; note that C2 is a high magnification of C1). High magnification picture shows that the cells that express high levels of Helios are negative or express low levels of NeuN (open arrows), while low Helios-expressing cells are positive for both markers (closed arrows) (C1). In addition, Helios is expressed by Bcl11b (Ctip2) (D) and Foxp1-positive neurons (E), two markers of striatal projection neurons. Scale bars: (A1, B1) 500 μm; (A2, B2, C, D, E) 100 μm.