FIG. 7.

Helios over-expression increases the levels of the neuronal marker β-III-tubulin. Over-expression of Helios in neurosphere cultures for 5 days induces an increase in the levels of the neuronal marker β-III-tubulin (Tuj-1). However, the expression of markers for neural precursors, vimentin and nestin, or glial cells, GFAP and Olig 1 and 2, are not modified after Helios over-expression in the neurosphere culture. Results are expressed as the mean of 5 neurosphere cultures and error bars represent the s.e.m. Statistical analysis was calculated by Student's t-test; **p<0.005 relative to neurospheres transduced with the control vector.