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. 2012 Mar 28;43(1):25. doi: 10.1186/1297-9716-43-25

Figure 1.

Figure 1

Schematic depicting the experimental design used in the current study. MDM were cultured in 24-well tissue culture plates and infected with M. avium subsp. paratuberculosis (MOI 2:1). RNA was extracted from infected (MPTb) and non-infected control MDM at three time points post-infection: 2 h, 6 h and 24 h. In addition, RNA was extracted from 0 h non-infected control MDM to assess potential non-experimental changes in gene expression. The MDM lysates from replicate tissue culture wells (shaded) were pooled for RNA extraction. Global gene expression for the non-infected control and infected MDM was analysed using the Affymetrix® GeneChip® Bovine Genome Array.