Table 1.
Comparison of fold-changes in gene expression in the infected MDM relative to the non-infected control MDM based on microarray and real time qRT-PCR results
| Gene symbol | Gene name | Gene description |
2 h mean fold-changes in gene expression |
6 h mean fold-changes in gene expression |
24 h mean fold-changes in gene expression |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Microarray data | Real time qRT-PCR | P-value (real time qRT-PCR) | Microarray data | Real time qRT-PCR | P-value (real time qRT-PCR) | Microarray data | Real time qRT-PCR | P-value (real time qRT-PCR) | |||
|
AREGB |
Amphiregulin B |
A growth-modulating glycoprotein |
NS |
- 1.29 |
≤ 0.01 |
- 2.03 |
- 2.58 |
≤ 0.05 |
NS |
- 1.54 |
≤ 0.01 |
|
CCL4 |
Chemokine (C-C motif) ligand 4 |
A proinflammatory and chemotactic chemokine |
+ 6.59 |
+ 28.87 |
≤ 0.001 |
+ 3.11 |
+ 9.67 |
≤ 0.05 |
NS |
+ 1.28 |
NS |
|
CCL5 |
Chemokine (C-C motif) ligand 5 |
A proinflammatory chemokine involved in the chemotaxis of monocytes and T-helper cells |
+ 3.56 |
+ 5.07 |
≤ 0.05 |
+ 4.02 |
+ 4.60 |
≤ 0.05 |
NS |
+ 2.11 |
≤ 0.05 |
|
CCL20 |
Chemokine (C-C motif) ligand 20 |
A chemokine involved in the chemoattraction of lymphocytes and neutrophils |
+ 10.47 |
+ 63.19 |
≤ 0.001 |
+ 2.80 |
+ 10.82 |
≤ 0.05 |
NS |
+ 1.22 |
NS |
|
CD40 |
CD40 molecule, TNF receptor superfamily member 5 |
A member of the TNF-receptor superfamily. Mediates the immune and inflammatory responses |
+ 4.44 |
+ 6.55 |
≤ 0.001 |
+ 5.23 |
+ 10.80 |
≤ 0.001 |
+ 1.71 |
+ 1.82 |
≤ 0.05 |
|
CFB |
Complement factor B |
A component of the alternative pathway of complement activation |
+ 3.54 |
+ 4.61 |
≤ 0.05 |
+ 4.84 |
+ 8.64 |
≤ 0.01 |
+ 2.98 |
+ 4.19 |
≤ 0.001 |
|
CXCL2 |
Chemokine (C-X-C motif) ligand 2 |
An immunoregulatory chemokine produced by activated monocytes and neutrophils at sites of inflammation |
+ 14.34 |
+ 28.56 |
≤ 0.001 |
+ 2.38 |
+ 3.07 |
≤ 0.01 |
NS |
+ 1.45 |
NS |
|
IL1B |
Interleukin 1, beta |
A cytokine that mediates the inflammatory response including cell proliferation, differentiation and apoptosis |
+ 14.41 |
+ 48.53 |
≤ 0.001 |
+ 2.97 |
+ 6.16 |
≤ 0.05 |
NS |
+ 2.25 |
≤ 0.05 |
|
IL6 |
Interleukin 6 |
A cytokine that functions in inflammation and the maturation of B cells |
+ 6.25 |
+ 21.12 |
≤ 0.001 |
NS |
+ 2.51 |
≤ 0.01 |
NS |
+ 1.38 |
NS |
|
IL15 |
Interleukin 15 |
A cytokine that regulates T and natural killer cell activation and proliferation |
+ 3.51 |
+ 4.40 |
≤ 0.001 |
+ 2.03 |
+ 2.73 |
≤ 0.01 |
NS |
1.04 |
NS |
|
IRF1 |
Interferon regulatory factor 1 |
A member of the interferon regulatory transcription factor family. An activator of interferon alpha and beta transcription |
+ 6.57 |
+ 12.64 |
≤ 0.001 |
+ 3.60 |
+ 5.20 |
≤ 0.01 |
NS |
+ 1.60 |
≤ 0.01 |
|
NFKB2 |
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) |
A pleiotropic transcription factor involved in inflammation, immunity, differentiation, cell growth and apoptosis |
+ 3.41 |
+ 5.37 |
≤ 0.001 |
+ 3.69 |
+ 5.82 |
≤ 0.001 |
NS |
+ 1.34 |
NS |
|
PIK3IP1 |
Phosphoinositide-3-kinase interacting protein 1 |
Suppresses the activity of phosphatidylinositol-3-kinase (PI3K), a regulator of cell division |
- 1.70 |
- 2.34 |
≤ 0.05 |
NS |
- 1.36 |
NS |
NS |
+ 1.54 |
NS |
|
SPRY2 |
Sprouty homolog 2 (Drosophila) |
An inhibitor of receptor tyrosine kinase signalling proteins |
- 2.07 |
- 2.20 |
NS |
NS |
- 1.12 |
NS |
NS |
- 1.46 |
NS |
| TNF | Tumor necrosis factor (TNF superfamily, member 2) | A proinflammatory cytokine (secreted by macrophages) involved in the regulation cell proliferation, differentiation and apoptosis, and coagulation. | + 13.89 | + 33.01 | ≤ 0.001 | + 2.73 | + 6.51 | ≤ 0.01 | NS | + 1.61 | ≤ 0.01 |
Geometric mean fold-changes of gene expression (infected MDM relative to non-infected control MDM from the same animal at each time point) are given for the microarray and real time qRT-PCR data at each time point. “NS” indicates that the gene was not significantly differentially expressed at the appropriate time point. The P-values obtained from analysis of the real time qRT-PCR analysis are presented. Geometric fold-changes given for the microarray data were significantly differentially expressed using an adjusted P-value ≤ 0.10. Descriptions of the function of each gene were obtained from the Entrez Gene database [44] and the GeneCards version 3 database [45].