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. 2012 Jul 13;10:30. doi: 10.1186/1477-3155-10-30

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Copyright ©2012 Arora et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterization of the purified HBcAg-EDIII-2 antigen. (A) The panel depicts VLPs formed by purified HBcAg-EDIII-2 protein (expressed in P.pastoris). Peaks 1 and 2 (shown in Figure 2B) were pooled, dialyzed, stained with 1% uranyl acetate and visualized under the electron microscope. (B) Electron microscopic visualization of the purified HBcAg carrier (expressed in E. coli). (C) Evaluation of the immunogenicity of the HBcAg-EDIII-2 antigen by ELISA. Antisera from mice immunized with the fusion antigen (solid red circles) and its precursors (HBcAg: empty black circles; EDIII-2: solid blue diamonds) were analyzed in an ELISA using recombinant EDIII-2 antigen (produced using P. pastoris) as the coating antigen.