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Evaluation of antibodies elicited by HBcAg-EDIII-2 fusion antigen by indirect immunofluorescence assay. Sera from mice that were mock-immunized (panel a), and immunized with HBcAg (panel b), EDIII-2 (panel c) or HBcAg-EDIII-2 (panel d) antigens were used as the primary antibody to pick up DENV-2 in infected BHK-21 cells, in conjunction with an anti-mouse IgG-FITC conjugate.
