Figure 2.

Identification of H-2^d-restricted epitopes in ZEBOV-GP.(A, B) BALB/c mice were immunized with Ad5-GPZ twice, at an interval of 4 weeks. Splenocytes were harvested 10 days after the second immunization and re-stimulated in vitro with the predicted peptides from ZEBOV-GP for use in the IFN-γ ELISPOT assay. GF and LV were observed to induce IFN-γ-specific spot forming. A negative control without peptide was included. (C, D) To further confirm the peptides identified in the ELISPOT assay and to compare the stimulation effect of GF, LV and EL, BALB/c mice were immunized with Ad5-GPZ or Ad5-EGFP (as a control) twice. Splenocytes were re-stimulated in vitro with GF, LV, EL or without peptides and the responding CD8⁺ T cells were visualized by intracellular IFN-γ staining.