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. 2012 Aug 9;7(8):e41466. doi: 10.1371/journal.pone.0041466

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© 2012 Anikeeva et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, B, C. Time-dependent changes in intracellular calcium concentration in CD8⁺68A62 CTL induced by indicated pHLA-A2 ligands assembled on either QD(520) (red) and Streptavidin (blue) scaffolds. Concentration of the probes in extracellular medium was the following: 1 nM for IV9-HLA-A2/QD(520) and IV9-HLA-A2/Streptavidin (A), 10 nM for A6-IV9-HLA-A2/QD(520) and A6-IV9-HLA-A2/Streptavidin (B), 5 nM for IV9-HLA-A2_mut/QD(520) and IV9-HLA-A2_mut/Streptavidin (C). Representative results are shown. D. Relative equilibrium binding of indicated pHLA-A2 ligands assembled on either QD(520) and Streptavidin scaffolds to 62A68 CD8⁺ CTL is shown. The relative amounts of cell-bound ligands were calculated from MFI measured by flow cytometry. Data represent mean ± s.d.