Figure 2. IL-2 induces CD47^high status on TCR-activated human CD47^low CD4 T cells.

(A) CFSE-labeled T_N cells (left panels) were activated with immobilized anti-CD3 and soluble anti-CD28 mAbs for 6 days. Unfractionated activated T cells or FACS sorted CD47^low T cells (middle panels) were restimulated for 5 days as indicated. CD47 expression in relation to cell division (CFSE cell dilution) and CCR7 expression in relation to CD47 status. (B) T_N were activated as in A and FACS sorted CD47^low (day 6) T cells were restimulated in presence of IL-2. Kinetics of CD47 (SIRP-α-Fc protein), CCR7 and CD127 expression is shown. (A–B) Data are representative of at least 4 to 6 independent experiments. Right panel A shows the mean ± standard deviation (SD) for 5 independent experiments. Student t test: *p<0.05.