Table 1. PE/PPE proteins selected for this study.
Rv number | PE/PPE | ESX associationa | # peptides (# pools) | M. bovis sequence comparison |
Rv0285 | PE5 | C | 12 (1) | Identical |
Rv0286 | PPE4 | C | 63 (6) | Identical |
Rv0915c | PPE14 | I | 52 (5) | Identical |
Rv0916c | PE7 | I | 11 (1) | Identical |
Rv1039c | PPE15 | P | 48 (5) | Identical |
Rv1040c | PE8 | P | 33 (3) | Identical |
Rv1168c | PPE17 | I | 42 (4) | Frameshift in M. bovis, otherwise identical |
Rv1169c | PE11 | I | 11 (1) | Identical |
Rv1195 | PE13 | P | 11 (1b) | Identical |
Rv1196 | PPE18 | P | 48 (5) | R235L, M249V, A275del, Q279E |
Rv1386 | PE15 | I | 12 (1) | Identical |
Rv1387 | PPE20 | I | 66 (6) | V94A |
Rv1787 | PPE25 | C | 45 (4) | S309del,V311G |
Rv1788 | PE18 | C | 11 (1) | Identical |
Rv1789 | PPE26 | C | 48 (5) | S274A |
Rv1790 | PPE27 | C | 10c (1) | P190S |
Rv1806 | PE20 | I | 11 (1) | Identical |
Rv1807 | PPE31 | I | 48 (5) | F223S, V234L |
Rv2107 | PE22 | I | 11 (1) | Identical |
Rv2108 | PPE36 | I | 29 (3) | Identical |
Rv2430c | PPE41 | I | 23 (2) | Identical |
Rv2431c | PE25 | I | 11 (1) | Identical |
Rv2768c | PPE43 | I | 47 (5) | P263R |
Rv2769c | PE27 | I | 33 (3) | P54S, V270M |
Rv3018A | PE27A | P | 1 (1b) | Identical |
Rv3018c | PPE46 | P | 19c (2) | Identical |
Rv3021c | PPE47 | P | 44 (1) | G222A, L240V, A242T |
Rv3022A | PE29 | P | 11c (1) | Identical |
Rv3477 | PE31 | I | 11 (1) | Identical |
Rv3478 | PPE60 | I | 48c (5) | Identical |
Rv3621c | PPE65 | P | 51 (5) | Deleted in M. bovis |
Rv3622c | PE32 | P | 11 (1) | Deleted in M. bovis |
Rv3872 | PE35 | C | 11 (1) | Identical |
Rv3873 | PPE68 | C | 45 (4) | Identical |
Rv3892c | PPE69 | C | 49 (5) | T19K |
Rv3893c | PE36 | C | 9 (1) | Identical |
a. C, PE/PPEs encoded by complete esx gene cluster; P, PE/PPE encoded by partial esx cluster; I, PE/PPE encoded by isolated pe/ppe pair (see Figure S1).
b. Single peptide for Rv3018A/PE27A mixed with PE13 set.
c. Pool does not include all possible overlapping peptides, some were excluded due to 100% identity.