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PiggyBac [3×p3 EGFP afm] was a basic transgenic vector with a report marker 3×P3-EGFP-SV40. PL and PR indicate the left and right terminal inverted repeats, respectively. 39 KP and A4P represent the 39 K promoter of BmNPV and A4 promoter of B. mori, respectively. Hr3 is an enhancer from BmNPV. Hycu-ep32 typifies the coding sequences of the hycu-ep32 gene, while SV40 indicates the polyadenylation signal.
