Figure 2. Tubulin acetylation does not affect microtubule velocity in kinesin-1 gliding assays.

(a) Schematic representation of the experimental setup for multi-motor gliding assays. We measured the velocities of short rhodamine-labeled acetylated and deacetylated microtubules propelled by surface-bound motors in presence of 1 mM ATP. The mean gliding velocities of 568 deacetylated microtubules (b) and 532 acetylated microtubules (c) did not differ significantly. The errors represent the SD of the Gaussian distributions.