Table 7. Effects of the ERK, JNK and pan-caspase inhibitors PD184352, TI-JIP153-163 and Z-VAD-fmk on NOD1 and TLR4 induced CXCL8 release in HMVEC.

Inhibitor	CXCL8 release (% responseto iE-DAP 10 µg/ml)	CXCL8 release (% responseto LPS 1 µg/ml)
PD184352 (1 µM)	124.7±25.6	103.9±6.1
TI-JIP153−163 (30 µM)	109.0±9.4	110.4±7.5
Z-VAD-fmk (10 µM)	93.1±7.3	103.4±13.5

HMVEC were cultured for 24 hours in 96 well plates with media alone (CTRL), iE-DAP 10 µg/ml (NOD1) or LPS 1 µg/ml (TLR4). Signalling inhibitors were added to the cells 30 minutes prior to the addition of agonists. Results are expressed as mean percentage release compared to that induced by the agonists alone (iE-DAP 10 µg/ml or LPS 1 µg/ml) ± SEM for n = 3. Results were analysed by one-sample t-test.