Table 1. Bacterial two-hybrid analysis investigating potential protein-protein interactions.

Target Protein (pTRG vector)
Bait Protein (pBT vector)	PA2571 RECdomain	PA2571 HKdomain	PA2571 Full length	PA2573 - TMdomain	PA2573 Fulllength	PA4781 REC domain
PA2572 REC domain	−	−	−	+	+	ND
PA2572 YN-GYP domain	−	−	−	−	−	ND
PA2572 Full length	−	−	−	+	+	ND
PA2573 - TM domain	ND	ND	ND	ND	ND	−

(+) indicates a positive protein-protein interaction based on growth of colonies on Selective Screening Media (SSM) (M9+ His-dropout media containing 5 mM 3-AT). (−) indicates a negative interaction based on lack of growth on SSM. (-TM) represents a PA2573 construct without the N-terminal transmembrane domains. Results were observations from three independent experiments.