Table 1. List of the quantified proteins in four biological replicates using the post-digest ICPL labeling methodology.
Proteins name | Rep 1 | Rep 2 | Rep 3 | Rep 4 | ||||||||
Ratio | SD | # Pept | Ratio | SD | # Pept | Ratio | SD | # Pept | Ratio | SD | # Pept | |
VAS14_02738 hypothetical protein | 0.58 | 0.00 | 1 | 0.57 | 0.00 | 1 | 0.51 | 1.25 | 2 | 0.71 | 0.00 | 1 |
VAS14_16916 phosphocarrier protein HPr | 0.62 | 1.33 | 3 | 0.67 | 1.11 | 5 | 0.54 | 1.21 | 6 | 0.44 | 1.20 | 5 |
VAS14_04158 arginine ABC transporter | 0.68 | 0.00 | 1 | 0.70 | 0.00 | 1 | 0.70 | 1.28 | 2 | 0.99 | 0.00 | 1 |
VAS14_22197 peptide ABC transporter | 0.72 | 1.04 | 3 | 0.67 | 1.16 | 2 | 0.59 | 1.32 | 2 | 0.75 | 1.20 | 6 |
VAS14_20236 phosphoglycerate kinase | 0.72 | 1.41 | 4 | 0.92 | 1.21 | 18 | 0.73 | 1.19 | 19 | 0.66 | 1.24 | 10 |
VAS14_17626 asparagine synthetase B | 0.73 | 1.31 | 6 | 0.72 | 1.20 | 9 | 0.80 | 1.16 | 16 | 1.01 | 1.32 | 12 |
VAS14_07409 carbamoyl-phosphate synthase small subunit | 0.74 | 1.55 | 4 | 0.81 | 1.37 | 5 | 0.85 | 1.20 | 6 | 0.79 | 1.16 | 3 |
VAS14_10219 phosphoenolpyruvate synthase | 0.77 | 1.25 | 9 | 0.81 | 1.31 | 20 | 0.84 | 1.24 | 26 | 0.89 | 1.24 | 21 |
VAS14_02983 putative Cold shock-like protein | 0.77 | 1.17 | 5 | 0.69 | 1.20 | 5 | 0.64 | 1.11 | 5 | 0.56 | 1.13 | 6 |
VAS14_17766 succinyl-CoA synthetase alpha subunit | 0.78 | 1.27 | 4 | 0.83 | 1.20 | 9 | 0.88 | 1.16 | 16 | 0.51 | 1.60 | 8 |
VAS14_07734 putative glutamate synthase, large subunit | 0.78 | 1.21 | 10 | 0.83 | 1.42 | 16 | 0.85 | 1.24 | 22 | 0.84 | 1.36 | 18 |
VAS14_21427 phosphoenolpyruvate carboxylase | 0.79 | 1.28 | 15 | 0.87 | 1.22 | 18 | 0.75 | 1.33 | 25 | 0.91 | 1.52 | 22 |
VAS14_14614 putative Cold shock-like protein | 0.79 | 1.13 | 4 | 0.75 | 1.20 | 4 | 0.81 | 1.10 | 4 | 0.59 | 1.06 | 3 |
VAS14_08175 3-isopropylmalate dehydrogenase | 0.80 | 1.10 | 5 | 0.77 | 1.27 | 6 | 0.82 | 1.45 | 8 | 0.81 | 1.26 | 4 |
VAS14_07404 carbamoyl-phosphate synthase large subunit | 0.80 | 1.08 | 12 | 0.86 | 1.39 | 18 | 0.87 | 1.16 | 13 | 0.88 | 1.23 | 15 |
VAS14_08340 inorganic pyrophosphatase | 0.84 | 1.30 | 6 | 0.80 | 1.22 | 6 | 0.78 | 1.14 | 7 | 0.86 | 1.26 | 8 |
VAS14_21527 triosephosphate isomerase | 0.84 | 1.13 | 4 | 0.71 | 1.09 | 7 | 0.84 | 1.15 | 7 | 0.71 | 1.32 | 6 |
VAS14_10584 3-deoxy-7-phosphoheptulonate synthase | 0.95 | 1.09 | 10 | 0.82 | 1.26 | 11 | 0.80 | 1.28 | 14 | 0.80 | 1.29 | 12 |
VAS14_05653 putative superoxide dismutase | 0.98 | 0.00 | 1 | 0.88 | 1.23 | 3 | 0.78 | 1.14 | 5 | 0.76 | 1.18 | 5 |
VAS14_18824 putative membrane protease subunits | 1.21 | 1.54 | 6 | 1.21 | 1.03 | 3 | 1.28 | 1.48 | 6 | 1.43 | 1.16 | 6 |
VAS14_07124 molecular chaperone DnaK | 1.24 | 1.23 | 25 | 1.14 | 1.24 | 29 | 1.06 | 1.27 | 27 | 0.89 | 1.28 | 25 |
VAS14_10439 putative ATP-dependent RNA helicase | 1.26 | 1.05 | 3 | 1.40 | 1.32 | 8 | 1.70 | 2.12 | 9 | 1.30 | 1.23 | 6 |
VAS14_12874 hypothetical outer membrane protein OmpA | 1.28 | 1.23 | 7 | 1.33 | 1.29 | 13 | 0.96 | 1.19 | 13 | 1.07 | 1.17 | 8 |
VAS14_06218 trigger factor | 1.31 | 1.23 | 17 | 1.19 | 1.24 | 27 | 1.18 | 1.22 | 30 | 1.15 | 1.21 | 29 |
VAS14_09799 glyoxalase family protein | 1.33 | 1.14 | 3 | 1.15 | 1.04 | 2 | 1.21 | 1.23 | 2 | 1.79 | 1.56 | 2 |
VAS14_05963 hypothetical outer membrane protein OmpA | 1.36 | 1.31 | 3 | 1.37 | 1.42 | 4 | 1.00 | 1.10 | 6 | 1.11 | 1.22 | 5 |
VAS14_07334 translation initiation factor IF-2 | 1.45 | 1.38 | 17 | 1.27 | 1.31 | 15 | 1.35 | 1.56 | 26 | 1.23 | 1.46 | 13 |
VAS14_18834 RNA-binding protein Hfq | 1.46 | 1.69 | 2 | 3.08 | 0.00 | 1 | 1.54 | 0.00 | 1 | 3.45 | 0.00 | 1 |
VAS14_00891 putative glutaredoxin 1 | 1.47 | 1.04 | 2 | 1.32 | 1.08 | 2 | 0.94 | 0.00 | 1 | 1.41 | 1.31 | 2 |
VAS14_06208 ATP-dependent protease ATP-binding subunit | 1.49 | 1.14 | 2 | 1.26 | 1.31 | 5 | 1.35 | 1.17 | 7 | 1.80 | 0.00 | 1 |
VAS14_21207 putative FKBP-type peptidyl-prolyl cis-trans isomerase 1 | 1.50 | 1.66 | 2 | 1.62 | 1.23 | 7 | 1.10 | 1.26 | 7 | 1.18 | 1.21 | 5 |
VAS14_07384 ompL_phopr porin-like protein L precursor | 1.56 | 1.11 | 3 | 0.96 | 1.22 | 3 | 1.35 | 1.18 | 5 | 1.76 | 1.27 | 3 |
VAS14_16369 putative GTP-binding protein | 1.64 | 1.47 | 3 | 1.32 | 1.17 | 3 | 1.53 | 1.17 | 2 | 1.45 | 1.18 | 2 |
VAS14_06783 putative antioxidant, AhpC/Tsa family protein | 1.65 | 1.37 | 17 | 1.67 | 1.24 | 20 | 1.41 | 1.15 | 17 | 1.58 | 1.16 | 21 |
VAS14_16756 Na(+)-translocating NADH-quinone reductase subunit F | 1.65 | 1.29 | 3 | 1.37 | 1.28 | 6 | 1.18 | 1.41 | 12 | 1.76 | 1.11 | 6 |
VAS14_14319 hypothetical protein | 1.81 | 0.00 | 1 | 1.70 | 1.34 | 2 | 1.79 | 1.06 | 2 | 1.57 | 1.17 | 3 |
VAS14_06728 putative inositol monophosphate family protein | 1.91 | 1.23 | 2 | 1.54 | 1.79 | 5 | 1.86 | 1.12 | 8 | 1.61 | 0.00 | 1 |
VAS14_11709 putative lipoprotein | 2.01 | 1.32 | 3 | 3.31 | 1.06 | 3 | 2.01 | 1.25 | 4 | 2.09 | 2.33 | 3 |
VAS14_07364 putative cell division protein FtsH | 2.32 | 1.26 | 4 | 2.28 | 1.23 | 5 | 1.07 | 1.22 | 10 | 1.66 | 1.19 | 7 |
VAS14_20496 recombinase A | 2.79 | 1.15 | 3 | 2.91 | 1.29 | 8 | 2.62 | 1.09 | 17 | 3.35 | 0.00 | 1 |
Proteins significantly different from the control within a given biological replicate are indicated with bold characters. Ratio: UVB/Dark, SD: standard deviation, #Pept: number of peptides used for protein quantification.