Table 2. Identification of the two-dimensionally separated protein spots of interest with the average fold change and associated p-values obtained from seven replicates.
Spot | Protein name | Ratio | p value (ANOVA) | # Peptides | Mass | Score | empAI |
4D | VAS14_05498 putative oligopeptide ABC transporter,periplasmic oligopeptide-binding protein | 0.34 | 3.70E-07 | 24 | 61324 | 1118 | 2.00 |
20D | VAS14_09609 hypothetical protein | 0.53 | 1.51E-04 | 7 | 13023 | 290 | 3.07 |
7D | VAS14_06013 glyceraldehyde-3-phosphate dehydrogenase | 0.53 | 7.12E-11 | 19 | 35441 | 2084 | 3.58 |
8D | VAS14_06013 glyceraldehyde-3-phosphate dehydrogenase | 0.53 | 7.12E-11 | 41 | 35441 | 2084 | 3.58 |
VAS14_14989 transaldolase | 5 | 35103 | 274 | 0.31 | |||
6D | VAS14_04158 arginine ABC transporter, periplasmic arginine-binding protein | 0.56 | 2.33E-06 | 15 | 26884 | 729 | 2.62 |
13D | VAS14_08125 pyruvate kinase | 0.57 | 1.94E-09 | 16 | 50710 | 946 | 1.13 |
VAS14_05498 putative oligopeptide ABC transporter,periplasmic oligopeptide-binding protein | 16 | 61324 | 534 | 0.97 | |||
15D | VAS14_00448 phosphoribosylaminoimidazole synthetase | 0.57 | 3.27E-07 | 12 | 37159 | 521 | 0.82 |
14D | VAS14_20441 phosphopyruvate hydratase | 0.60 | 4.79E-07 | 79 | 45977 | 4993 | 7.59 |
9D | VAS14_10584 3-deoxy-7-phosphoheptulonate synthase | 0.60 | 1.07E-06 | 17 | 38616 | 1119 | 2.16 |
VAS14_20241 fructose-bisphosphate aldolase | 4 | 38792 | 165 | 0.28 | |||
16D | VAS14_20441 phosphopyruvate hydratase | 0.66 | 6.49E-04 | 2 | 45977 | 50 | 0.07 |
19D | VAS14_00681 bacterioferritin comigratory protein | 0.67 | 3.40E-07 | 11 | 17417 | 163 | 1.43 |
2D | VAS14_19086 bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase | 0.67 | 1.37E-08 | 11 | 57424 | 674 | 0.88 |
VAS14_18544 30S ribosomal protein S1 | 8 | 60777 | 544 | 0.52 | |||
VAS14_17821 arginyl-tRNA synthetase | 7 | 64732 | 474 | 0.48 | |||
3D | VAS14_22197 peptide ABC transporter, periplasmic peptide-binding protein | 0.70 | 2.77E-06 | 10 | 57916 | 480 | 0.39 |
11D | VAS14_16581 serine hydroxymethyltransferase | 0.70 | 9.94E-09 | 24 | 45287 | 609 | 1.68 |
12D | VAS14_16581 serine hydroxymethyltransferase | 0.71 | 7.30E-08 | 5 | 45287 | 422 | 0.33 |
VAS14_18544 30S ribosomal protein S1 | 4 | 60777 | 246 | 0.17 | |||
VAS14_06013 glyceraldehyde-3-phosphate dehydrogenase | 2 | 35441 | 146 | 0.2 | |||
VAS14_22994 glutamine synthetase | 3 | 51536 | 121 | 0.2 | |||
18D | VAS14_21527 triosephosphate isomerase | 0.71 | 2.55E-10 | 8 | 26968 | 374 | 0.59 |
1D | VAS14_14099 16 kDa heat shock protein A | 0.75 | 2.95E-06 | 4 | 16561 | 325 | 1.11 |
10D | VAS14_20756 putative 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase | 0.75 | 1.38E-08 | 15 | 36213 | 369 | 1.4 |
21D | VAS14_05668 aspartate-semialdehyde dehydrogenase | 0.75 | 9.39E-06 | 13 | 40791 | 513 | 1.18 |
VAS14_08310 malate dehydrogenase | 2 | 32486 | 212 | 0.21 | |||
22D | VAS14_12994 glycine cleavage system protein T2 | 0.76 | 2.85E-04 | 6 | 40822 | 311 | 0.6 |
VAS14_09604 acetyl-CoA acetyltransferase | 6 | 41768 | 316 | 0.46 | |||
5D | VAS14_20221 S-adenosylmethionine synthetase | 0.79 | 3.77E-05 | 2 | 42360 | 124 | 0.16 |
VAS14_05498 putative oligopeptide ABC transporter,periplasmic oligopeptide-binding protein | 4 | 61324 | 180 | 0.10 | |||
17D | VAS14_08310 malate dehydrogenase | 0.79 | 4.67E-06 | 53 | 32486 | 2873 | 7.49 |
3U | VAS14_19241 30S ribosomal protein S3 | 1.26 | 5.38E-06 | 2 | 25439 | 86 | 0.28 |
VAS14_02743 hypothetical protein | 2 | 30191 | 67 | 0.11 | |||
VAS14_20076 3-methyl-2-oxobutanoate hydroxymethyltransferase | 2 | 28835 | 58 | 0.12 | |||
4U | VAS14_19236 50S ribosomal protein L22 | 1.26 | 2.73E-05 | 3 | 12172 | 46 | 0.28 |
11U | VAS14_16921 putative cysteine synthase A | 1.33 | 1.55E-06 | 7 | 34364 | 348 | 0.74 |
VAS14_09694 hypothetical ABC transporter, substrate binding protein | 11 | 36644 | 226 | 0.68 | |||
12U | VAS14_11709 putative lipoprotein | 1.33 | 1.48E-05 | 11 | 9172 | 204 | 1.62 |
13U | VAS14_11709 putative lipoprotein | 1.33 | 6.75E-06 | 4 | 9172 | 234 | 1.62 |
9U | VAS14_18941 chaperonin GroEL | 1.38 | 5.27E-07 | 5 | 57359 | 234 | 0.25 |
VAS14_22517 bifunctional N-acetylglucosamine-1-phosphate uridyltransferase | 5 | 48395 | 102 | 0.12 | |||
8U | VAS14_20971 ribosome releasing factor | 1.40 | 2.35E-08 | 8 | 20820 | 431 | 1.45 |
15U | VAS14_19006 acetyl-coenzyme A synthetase | 1.41 | 5.73E-05 | 8 | 72060 | 556 | 0.2 |
5U | VAS14_19236 50S ribosomal protein L22 | 1.50 | 9.95E-04 | 2 | 12172 | 118 | 0.28 |
6U | VAS14_08110 hypothetical protein | 1.60 | 4.07E-07 | 2 | 16134 | 137 | 0.47 |
7U | VAS14_19181 transcription antitermination protein NusG | 1.61 | 1.27E-06 | 4 | 16895 | 218 | 1.08 |
VAS14_20971 ribosome releasing factor | 2 | 20820 | 65 | 0.16 | |||
2U | VAS14_00453 uracil phosphoribosyltransferase | 1.79 | 5.24E-04 | 2 | 22712 | 77 | 0.32 |
VAS14_06783 putative antioxidant, AhpC/Tsa family protein | 3 | 22576 | 48 | 0.32 | |||
10U | VAS14_07384 ompL_phopr porin-like protein L precursor | 1.86 | 5.08E-06 | 25 | 37973 | 1438 | 4.79 |
1U | VAS14_19811 stringent starvation protein A | 2.66 | 2.62E-09 | 3 | 24257 | 133 | 0.47 |
VAS14_06783 putative antioxidant, AhpC/Tsa family protein | 7 | 22576 | 118 | 0.32 | |||
14U | VAS14_12129 NADH:flavin oxidoreductase | 3.29 | 1.04E-06 | 11 | 40302 | 434 | 0.61 |
VAS14_20496 recombinase A | 4 | 37902 | 78 | 0.12 |
The ratio was determined by comparing the peak intensities in LC-MS runs of UVB versus dark conditions (UVB/Dark). The number of peptides assigned a Mascot score above 35, as well as the protein mass, score and empAI value, are also listed for each identified protein. (D: down-regulated; U: up-regulated).