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. 2012 Aug 1;7(8):e42299. doi: 10.1371/journal.pone.0042299

Table 2. Identification of the two-dimensionally separated protein spots of interest with the average fold change and associated p-values obtained from seven replicates.

Spot Protein name Ratio p value (ANOVA) # Peptides Mass Score empAI
4D VAS14_05498 putative oligopeptide ABC transporter,periplasmic oligopeptide-binding protein 0.34 3.70E-07 24 61324 1118 2.00
20D VAS14_09609 hypothetical protein 0.53 1.51E-04 7 13023 290 3.07
7D VAS14_06013 glyceraldehyde-3-phosphate dehydrogenase 0.53 7.12E-11 19 35441 2084 3.58
8D VAS14_06013 glyceraldehyde-3-phosphate dehydrogenase 0.53 7.12E-11 41 35441 2084 3.58
VAS14_14989 transaldolase 5 35103 274 0.31
6D VAS14_04158 arginine ABC transporter, periplasmic arginine-binding protein 0.56 2.33E-06 15 26884 729 2.62
13D VAS14_08125 pyruvate kinase 0.57 1.94E-09 16 50710 946 1.13
VAS14_05498 putative oligopeptide ABC transporter,periplasmic oligopeptide-binding protein 16 61324 534 0.97
15D VAS14_00448 phosphoribosylaminoimidazole synthetase 0.57 3.27E-07 12 37159 521 0.82
14D VAS14_20441 phosphopyruvate hydratase 0.60 4.79E-07 79 45977 4993 7.59
9D VAS14_10584 3-deoxy-7-phosphoheptulonate synthase 0.60 1.07E-06 17 38616 1119 2.16
VAS14_20241 fructose-bisphosphate aldolase 4 38792 165 0.28
16D VAS14_20441 phosphopyruvate hydratase 0.66 6.49E-04 2 45977 50 0.07
19D VAS14_00681 bacterioferritin comigratory protein 0.67 3.40E-07 11 17417 163 1.43
2D VAS14_19086 bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase 0.67 1.37E-08 11 57424 674 0.88
VAS14_18544 30S ribosomal protein S1 8 60777 544 0.52
VAS14_17821 arginyl-tRNA synthetase 7 64732 474 0.48
3D VAS14_22197 peptide ABC transporter, periplasmic peptide-binding protein 0.70 2.77E-06 10 57916 480 0.39
11D VAS14_16581 serine hydroxymethyltransferase 0.70 9.94E-09 24 45287 609 1.68
12D VAS14_16581 serine hydroxymethyltransferase 0.71 7.30E-08 5 45287 422 0.33
VAS14_18544 30S ribosomal protein S1 4 60777 246 0.17
VAS14_06013 glyceraldehyde-3-phosphate dehydrogenase 2 35441 146 0.2
VAS14_22994 glutamine synthetase 3 51536 121 0.2
18D VAS14_21527 triosephosphate isomerase 0.71 2.55E-10 8 26968 374 0.59
1D VAS14_14099 16 kDa heat shock protein A 0.75 2.95E-06 4 16561 325 1.11
10D VAS14_20756 putative 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase 0.75 1.38E-08 15 36213 369 1.4
21D VAS14_05668 aspartate-semialdehyde dehydrogenase 0.75 9.39E-06 13 40791 513 1.18
VAS14_08310 malate dehydrogenase 2 32486 212 0.21
22D VAS14_12994 glycine cleavage system protein T2 0.76 2.85E-04 6 40822 311 0.6
VAS14_09604 acetyl-CoA acetyltransferase 6 41768 316 0.46
5D VAS14_20221 S-adenosylmethionine synthetase 0.79 3.77E-05 2 42360 124 0.16
VAS14_05498 putative oligopeptide ABC transporter,periplasmic oligopeptide-binding protein 4 61324 180 0.10
17D VAS14_08310 malate dehydrogenase 0.79 4.67E-06 53 32486 2873 7.49
3U VAS14_19241 30S ribosomal protein S3 1.26 5.38E-06 2 25439 86 0.28
VAS14_02743 hypothetical protein 2 30191 67 0.11
VAS14_20076 3-methyl-2-oxobutanoate hydroxymethyltransferase 2 28835 58 0.12
4U VAS14_19236 50S ribosomal protein L22 1.26 2.73E-05 3 12172 46 0.28
11U VAS14_16921 putative cysteine synthase A 1.33 1.55E-06 7 34364 348 0.74
VAS14_09694 hypothetical ABC transporter, substrate binding protein 11 36644 226 0.68
12U VAS14_11709 putative lipoprotein 1.33 1.48E-05 11 9172 204 1.62
13U VAS14_11709 putative lipoprotein 1.33 6.75E-06 4 9172 234 1.62
9U VAS14_18941 chaperonin GroEL 1.38 5.27E-07 5 57359 234 0.25
VAS14_22517 bifunctional N-acetylglucosamine-1-phosphate uridyltransferase 5 48395 102 0.12
8U VAS14_20971 ribosome releasing factor 1.40 2.35E-08 8 20820 431 1.45
15U VAS14_19006 acetyl-coenzyme A synthetase 1.41 5.73E-05 8 72060 556 0.2
5U VAS14_19236 50S ribosomal protein L22 1.50 9.95E-04 2 12172 118 0.28
6U VAS14_08110 hypothetical protein 1.60 4.07E-07 2 16134 137 0.47
7U VAS14_19181 transcription antitermination protein NusG 1.61 1.27E-06 4 16895 218 1.08
VAS14_20971 ribosome releasing factor 2 20820 65 0.16
2U VAS14_00453 uracil phosphoribosyltransferase 1.79 5.24E-04 2 22712 77 0.32
VAS14_06783 putative antioxidant, AhpC/Tsa family protein 3 22576 48 0.32
10U VAS14_07384 ompL_phopr porin-like protein L precursor 1.86 5.08E-06 25 37973 1438 4.79
1U VAS14_19811 stringent starvation protein A 2.66 2.62E-09 3 24257 133 0.47
VAS14_06783 putative antioxidant, AhpC/Tsa family protein 7 22576 118 0.32
14U VAS14_12129 NADH:flavin oxidoreductase 3.29 1.04E-06 11 40302 434 0.61
VAS14_20496 recombinase A 4 37902 78 0.12

The ratio was determined by comparing the peak intensities in LC-MS runs of UVB versus dark conditions (UVB/Dark). The number of peptides assigned a Mascot score above 35, as well as the protein mass, score and empAI value, are also listed for each identified protein. (D: down-regulated; U: up-regulated).