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. 2012 Aug 3;7(8):e41614. doi: 10.1371/journal.pone.0041614

Figure 4. Estrogen altered the expression of cyclin D1.

Figure 4

Estrogen reduced the expression of cyclin D1 in WT-SMCs (A) but up-regulated it in CSE-KO-SMCs (B). The cells were treated with 100 nM estrogen for 72 h. * p<0.05. C, Knockdown of ERα reversed estrogen-decreased expression of cyclin D1 in WT-SMCs. After transfection with siRNA ERα or siRNA C at 50 nM for 12 h, the cells were incubated with 100 nM estrogen for another 36 h. * p<0.05 vs. siRNA C group; # p<0.05 vs. siRNA C or siRNA C+estrogen group. D, Overexpression of ERα reversed estrogen-increased expression of cyclin D1 in CSE-KO-SMCs. After transfection with pEGFP-C1-ERα plasmid or control plasmid for 24 h, the cells were incubated with 100 nM estrogen for another 48 h. * p<0.05 vs. control vector group; # p<0.05 vs. control vector or control vector+estrogen group. All data were from three independent experiments.